[Features of mutated changes of genomic RNA of cold-adapted and hr-variants of influenza group A virus, detected by RNA:RNA hybridization].
Identifieur interne : 002065 ( Main/Exploration ); précédent : 002064; suivant : 002066[Features of mutated changes of genomic RNA of cold-adapted and hr-variants of influenza group A virus, detected by RNA:RNA hybridization].
Auteurs : A I Klimov ; V V IotovSource :
- Molekuliarnaia genetika, mikrobiologiia i virusologiia [ 0208-0613 ] ; 1990.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : RNA, Double-Stranded, RNA, Messenger, RNA, Viral.
- chemical : Antigens, Viral, Epitopes.
- genetics : Influenza A virus.
- immunology : Influenza A virus.
- Cold Temperature, Electrophoresis, Polyacrylamide Gel, Genes, Viral, Mutation, Nucleic Acid Hybridization.
Abstract
The presence of mutations in the majority of the genes of cold-adapted strains A/Leningrad/134/17/57 (H2N2), A/Leningrad/134/47/57 (H2N2) and A/PR/8/59/1 (H1N1) of influenza A virus has been demonstrated by the RNA-RNA hybridization with the subsequent electrophoresis of double-stranded RNA in 7.5% polyacrylamide gel. The strains were cultivated 17, 47 and 59 passages in the chicken embryos at 25 degrees C. In the genomes of variants passaged in chicken embryos at optimal temperature of incubation 36 degrees C (hr-variants) the used technique permits identification of a single mutant gene. The obtained data suppose the attenuation of cold-adapted vaccine strains of influenza A virus and their high genetic stability to be a result of selection of the variants obtaining multiple mutations in the genome during passaging of the virions at cold temperature. The attenuation of hr-variants is defined by 1-2 mutations (first of all in HA-gene) that makes understandable their inability to serve as donors for recombinant live influenza vaccines construction.
PubMed: 1706066
Affiliations:
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Le document en format XML
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The strains were cultivated 17, 47 and 59 passages in the chicken embryos at 25 degrees C. In the genomes of variants passaged in chicken embryos at optimal temperature of incubation 36 degrees C (hr-variants) the used technique permits identification of a single mutant gene. The obtained data suppose the attenuation of cold-adapted vaccine strains of influenza A virus and their high genetic stability to be a result of selection of the variants obtaining multiple mutations in the genome during passaging of the virions at cold temperature. The attenuation of hr-variants is defined by 1-2 mutations (first of all in HA-gene) that makes understandable their inability to serve as donors for recombinant live influenza vaccines construction.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Iotov, V V" sort="Iotov, V V" uniqKey="Iotov V" first="V V" last="Iotov">V V Iotov</name><name sortKey="Klimov, A I" sort="Klimov, A I" uniqKey="Klimov A" first="A I" last="Klimov">A I Klimov</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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